Chemiluminescence analysis of the activity of Pecten maximus hemocytes stimulated with zymosan and host-specific Rickettsiales-like organisms
نویسنده
چکیده
During a study of Rickettsiales-like organisms (RLO) in Pecten maximus gill, histological observation of free RLO in hemolymph led us to consider interactions between the pathogen and the hemocytes. Firstly, the production of oxygen radicals by hemocytes was established using a luminolenhanced chemiluminescence method with zymosan as stimulant. The effects of several inh~bitors of oxidative metabolism were investigated. The internalization of RLO into hemocytes was demonstrated by electron microscopy but was not related to chemiluminescent activity. The possible involvement of RLO acid phosphatase as the inhibitor of respiratory burst was assessed.
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